| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Northwest (Cedar Hill) High Scho | A | Grellner | Wilson | Holland | Bounds | 3:52.59 | Verified |
| 4 | Notre Dame (Cape Girardeau) High | A | Brown | Pfeifer | Dow | Bruenderman | 3:50.75 | Verified |
| 5 | Jackson High School | A | CAIN | Gross | Brazel | Boyd | 3:52.00 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Riverview Gardens High School | A | Abel | Carlock | Crymes | Davis | 3:43.80 | Verified |
| 3 | Kirkwood High School | A | Welch | Patterson | Hickman | Baker | 3:35.00 | Unverified: |
| 4 | Lafayette (Wildwood) High School | A | Wildt | Aven | Ems | Malek | 3:31.00 | Unverified: |
| 5 | Hazelwood Central High School | A | Johnson | Anthony | Hunter | Harris | 3:33.94 | Verified |
| 6 | Marquette High School | A | CODY-JACKSON | Brown | Jones | WILLIAMS | 3:37.49 | Verified |
| 7 | Parkway West High School | A | Harris | Moore | Hathaway | Hawver | 3:48.69 | Verified |