| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Parkway North High School | A | McDevitt | Shields | Ryan | Mendez | 4:28.63 | Verified |
| 4 | Marquette High School | A | NOLAN | JONES | TERRY | KOSSMANN | 3:54.00 | Unverified: Lindbergh Boys Flyer Track and F |
| 5 | Jackson High School | A | Lacey | Bell | Craigmiles | Sterling | 3:58.00 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Parkway West High School | A | Parini | Carevic | Feng | Cummins | 3:51.00 | Unverified: |
| 3 | Hazelwood Central High School | A | Chambers | Logan | McDaniel | Nance | 3:43.94 | Unverified: |
| 4 | Francis Howell North High School | A | Fifer | Freeman | Lin | Engelhard | 3:39.81 | Verified |
| 5 | Lafayette (Wildwood) High School | A | Parks | Preckel | KING | STITH | 3:42.24 | Unverified: |
| 6 | Kirkwood High School | A | Reynolds | Birmingham | Boswinkel | Stevener | 3:48.00 | Unverified: |
| 7 | Northwest (Cedar Hill) High Scho | A | Washington | Porter | Riley | Campbell | 3:52.59 | Verified |