| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Oakville High School | A | Witte | Beidle | WETTEROFF | JONAS | 4:54.00 | Unverified: Affton Invite |
| 2 | Lafayette (Wildwood) High School | A | Pauls | HALTER | MOULTON | Singh | 4:31.87 | Verified |
| 3 | Northwest (Cedar Hill) High Scho | A | Brown | Wheeler | Ryan | Bertani | 4:30.00 | Unverified: Kirkwood Early Bird |
| 4 | Eureka High School | A | DEAKIN | Grobe | Gentilini | Crooks | 4:20.96 | Verified |
| 5 | Parkway West High School | A | Whitaker | Grunzinger | Drake | Yates | 4:30.00 | Unverified: |
| 6 | Hazelwood Central High School | A | Ray | Brown | Mckenzie | Mapp | 4:30.27 | Verified |
| 7 | Marquette High School | A | TROUPE | Arnold | DAVIS | EHIMARE | 4:34.03 | Verified |
| 8 | Lindbergh High School | A | Adams | Arling | Doss | Forbes | NT | Unverified: |