| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lindbergh High School | A | Bani | Gonzalez | Hobson | Soto | NT | Unverified: |
| 2 | Hazelwood Central High School | A | Simmons | Elam | Catching | Williams | 4:50.27 | Unverified: |
| 3 | Jackson High School | A | GROSS | McBryde | Pope | Golinski | 4:43.00 | Unverified: |
| 4 | Kirkwood High School | A | McClure | Cornelius | Person | Martz | 4:40.00 | Unverified: |
| 5 | Eureka High School | A | Conley | BRICKMAN | Morse | Christensen | 4:42.00 | Unverified: |
| 6 | Lafayette (Wildwood) High School | A | JOHNSON | Mimlitz | GAINES | POLK | 4:45.00 | Unverified: |
| 7 | Northwest (Cedar Hill) High Scho | A | Wood | Ziegler | Orozco | Cronin | 4:53.00 | Unverified: Kirkwood Early Bird |
| 8 | Marquette High School | A | Walters | PILLAI | BALLARD | JESPERSON | 4:55.00 | Unverified: |