| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lafayette (Wildwood) High School | A | Compton | Miller | Aven | Malek | 8:14.60 | Verified |
| 2 | Jackson High School | A | Brazel | Gentry | Puls | Wendel | 8:20.95 | Verified |
| 3 | Kirkwood High School | A | Patterson | Baker | Lee | Hickman | 8:30.50 | Unverified: |
| 4 | Parkway West High School | A | Patten | Kallaos | Samal | Hathaway | 8:45.00 | Unverified: |
| 5 | Northwest (Cedar Hill) High Scho | A | Grellner | Wilson | Barnhart | Glassner | 8:53.80 | Verified |
| 6 | Francis Howell North High School | A | Alexander | Brewer | McGuire | Jefferson | 8:55.77 | Verified |
| 7 | Marquette High School | A | LINGAFELTER | Hummel | Winka | Kilo | 8:57.40 | Verified |
| 8 | Hazelwood Central High School | A | Quinn | Godfrey | Webster | Ellis | 8:59.61 | Verified |
| 9 | Riverview Gardens High School | A | Abel | Crymes | Davis | Love | 9:14.18 | Verified |
| 10 | Notre Dame (Cape Girardeau) High | A | Givens | Coalter | Schnurbusch | Morris | 9:15.80 | Verified |