| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HOOKS, Eryn | 9 | McCluer High School | NT | Unverified: |
| 4 | GILLS, Ashten | JR | Parkway West High School | 31.49 | Verified |
| 5 | SYKES, Myrakal | 9 | McCluer High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JONAS, MADYSEN | SO | Oakville High School | 31.06 | Unverified: Affton |
| 2 | HUCK, Lainey | 9 | Notre Dame (Cape Girardeau) High | 30.76 | Verified |
| 3 | MUELLER, Jenna | JR | Oakville High School | 30.20 | Unverified: Affton |
| 4 | MCGEE, Lindsey | SR | Marquette High School | 29.50 | Unverified: |
| 5 | WHITE, Melissa | JR | Lafayette (Wildwood) High School | 29.51 | Verified |
| 6 | TATUM, Courtney | 9 | Notre Dame (Cape Girardeau) High | 30.37 | Verified |
| 7 | JEFFERSON, Eryonne | SR | Hazelwood Central High School | 30.87 | Verified |
| 8 | CALLIS, AnnaJane | SO | Jackson High School | 31.33 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | POWELL, Emma | JR | Jackson High School | 29.40 | Verified |
| 2 | WILKINS, Janiya | SO | Hazelwood Central High School | 29.25 | Verified |
| 3 | JOHNSON, Nykira | SO | Parkway West High School | 29.10 | Unverified: |
| 4 | BEAUMONT, Jessie | JR | Kirkwood High School | 28.94 | Verified |
| 5 | BROWN, Samantha | SR | Northwest (Cedar Hill) High Scho | 29.00 | Unverified: Kirkwood Early Bird |
| 6 | CHANCE, Lauren | SO | Francis Howell North High School | 29.20 | Unverified: |
| 7 | SHELLEDY, Isabella | 9 | Northwest (Cedar Hill) High Scho | 29.30 | Unverified: Kirkwood Early Bird |
| 8 | SEITZ, MADELYN | JR | Eureka High School | 29.43 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NELSON GRIMES, Carmon | JR | Parkway North High School | 28.57 | Verified |
| 2 | JONES, Amani | SR | Francis Howell North High School | 28.28 | Verified |
| 3 | TROUPE, JOSHLYN | JR | Marquette High School | 27.80 | Unverified: |
| 4 | DAVIS, Chloe | SR | Parkway North High School | 27.33 | Verified |
| 5 | BROWN, Avery | 9 | Lafayette (Wildwood) High School | 27.50 | Unverified: |
| 6 | WILKES, Mariah | SO | Kirkwood High School | 27.87 | Verified |
| 7 | SCHWEIZER, Kaitlyn | SR | Eureka High School | 28.30 | Unverified: |
| 8 | HIERS, Hannah | JR | Lindbergh High School | 28.78 | Verified |