| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HOUSE, Tori | 9 | McCluer High School | NT | Unverified: |
| 4 | HAVILAND, Kathleen | JR | Lindbergh High School | NT | Unverified: |
| 5 | MOORE, Kiara | SO | Hazelwood Central High School | NT | Unverified: |
| 6 | GARDNER, Emma | SO | Lindbergh High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MAYES, Cherish | 9 | Hazelwood Central High School | NT | Unverified: |
| 2 | TABRON, Demelliah | SR | Riverview Gardens High School | NT | Unverified: |
| 3 | PAGE, Claire | 9 | Notre Dame (Cape Girardeau) High | 34.65 | Verified |
| 4 | CULBERT, Peyton | JR | Parkway West High School | 33.50 | Unverified: |
| 5 | HUFF, Heidi | 9 | Francis Howell North High School | 33.70 | Unverified: |
| 6 | COLLINS, Hunter | 9 | Notre Dame (Cape Girardeau) High | 36.56 | Verified |
| 7 | ROBINSON, Da'Niya | 9 | McCluer High School | NT | Unverified: |
| 8 | TILLMAN, Alaina | 9 | Parkway North High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | POPP, SYDNEY | 9 | Jackson High School | 32.70 | Unverified: |
| 2 | DAVIS, Makaila | SO | Parkway North High School | 32.46 | Verified |
| 3 | CRONIN, Lauren | 9 | Northwest (Cedar Hill) High Scho | 32.30 | Unverified: Kirkwood Early Bird |
| 4 | SOLOMAKHINA, KARINA | JR | Marquette High School | 31.80 | Unverified: |
| 5 | POLK, DESTINY | JR | Lafayette (Wildwood) High School | 32.00 | Unverified: |
| 6 | AMOS, Briana | 9 | Northwest (Cedar Hill) High Scho | 32.40 | Unverified: Kirkwood Early Bird |
| 7 | SHIPMAN, Claire | SR | Jackson High School | 32.50 | Unverified: |
| 8 | HUFF, Olivia | 9 | Francis Howell North High School | 33.40 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SANDERS, Samari | SO | Parkway West High School | 31.10 | Unverified: |
| 2 | BUNCH, SIERRA | 9 | Marquette High School | 30.50 | Unverified: |
| 3 | WRIGHT, Amelia | SO | Kirkwood High School | 29.50 | Verified |
| 4 | DOSS, MADISON | 9 | Eureka High School | 28.98 | Verified |
| 5 | CORNELIUS, Khamori | SR | Kirkwood High School | 29.04 | Verified |
| 6 | WILDT, KARA | 9 | Lafayette (Wildwood) High School | 30.00 | Unverified: |
| 7 | FRAZIER, INDYA | 9 | Eureka High School | 31.06 | Verified |
| 8 | RICHARDSON, Alana | SR | Riverview Gardens High School | 31.27 | Verified |