| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PROUTY, Quinn | SO | Lindbergh High School | NT | Unverified: |
| 2 | HUNT, Jaylin | 9 | Oakville High School | NT | Unverified: |
| 3 | GLADDEN, Mykale | JR | Riverview Gardens High School | NT | Unverified: |
| 4 | GRIFFIN, J'Kyla | JR | Northwest (Cedar Hill) High Scho | 1:03.00 | Unverified: Kirkwood Early Bird |
| 5 | SHANK, Jojo | SO | Parkway West High School | 1:08.00 | Unverified: |
| 6 | ISLAM, SABRINA | SO | Oakville High School | NT | Unverified: |
| 7 | GAINER, Micaiah | SR | Lindbergh High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CONSOLINO, Rose | 9 | Northwest (Cedar Hill) High Scho | 1:01.00 | Unverified: Kirkwood Early Bird |
| 2 | PERSON, Laila | SO | Kirkwood High School | 59.01 | Verified |
| 3 | MCCLURE, Alli | SO | Kirkwood High School | 54.49 | Verified |
| 4 | WILLIAMS, Kennedy | 9 | Hazelwood Central High School | 45.01 | Verified |
| 5 | WITHERSPOON, Stevie | 9 | Hazelwood Central High School | 52.40 | Unverified: |
| 6 | WEISS, MAKENNA | SO | Lafayette (Wildwood) High School | 55.62 | Unverified: |
| 7 | DOHERTY, GRACE | 9 | Lafayette (Wildwood) High School | 1:00.54 | Verified |
| 8 | HOWARD, AUTUMN | 9 | Jackson High School | 1:01.54 | Verified |