| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | CODY-JACKSON, CALEB | JR | Marquette High School | NT | Unverified: |
| 3 | JONES, Dion | JR | Hazelwood Central High School | NT | Unverified: |
| 4 | COLEMAN, Darrion | SR | Northwest (Cedar Hill) High Scho | NT | Unverified: |
| 5 | MOSLEY, Donnell | SO | McCluer High School | NT | Unverified: |
| 6 | VOB ORB, Calvin | SO | Francis Howell North High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BROWN, Derrick | SR | Marquette High School | NT | Unverified: |
| 2 | POLLARD, Jeremiah | SR | Francis Howell North High School | 1:01.06 | Verified |
| 3 | GROSS, Layne | JR | Jackson High School | 58.23 | Verified |
| 4 | WELCH, Jaylon | JR | Kirkwood High School | 55.70 | Unverified: |
| 5 | CRYMES, Chad | SR | Riverview Gardens High School | 56.00 | Unverified: |
| 6 | SMITH, Aleczander | JR | Lafayette (Wildwood) High School | 59.00 | Unverified: |
| 7 | STIEGEMEYER, Aiden | 9 | Notre Dame (Cape Girardeau) High | 1:02.25 | Verified |
| 8 | PARKER, Dallas | SR | Parkway North High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ABEL, Dennis | SO | Riverview Gardens High School | 54.75 | Unverified: |
| 2 | PFEIFER, Emmett | SO | Notre Dame (Cape Girardeau) High | 54.00 | Unverified: |
| 3 | SLEDGE, Melvin | 9 | Hazelwood East High School | 53.52 | Verified |
| 4 | COMPTON, Brycen | JR | Lafayette (Wildwood) High School | 52.20 | Unverified: |
| 5 | GRELLNER, Justin | SR | Northwest (Cedar Hill) High Scho | 53.29 | Verified |
| 6 | HARRIS, Micah | SR | Parkway West High School | 53.70 | Unverified: |
| 7 | MCCOY, Carl | JR | McCluer High School | 54.07 | Verified |
| 8 | CAIN, BLAKE | 9 | Jackson High School | 55.53 | Verified |