| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | KOTTMEIER, Skyler | JR | Lindbergh High School | NT | Unverified: |
| 4 | WILLIAMS, Roniya | JR | McCluer High School | NT | Unverified: |
| 5 | WAFFORD, Raina | JR | Hazelwood Central High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | UNION, Journey | JR | Hazelwood Central High School | NT | Unverified: |
| 3 | SYKES, Myrakal | 9 | McCluer High School | 1:12.34 | Verified |
| 4 | DAFFRON, HEIDI | 9 | Eureka High School | 1:11.00 | Unverified: |
| 5 | WITTE, Olivia | SR | Oakville High School | 1:11.00 | Unverified: Affton |
| 6 | WETTEROFF, Samantha | SO | Oakville High School | 1:17.00 | Unverified: Affton |
| 7 | DRAKE, Makinsey | JR | Parkway West High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | EHIMARE, OMOYE | JR | Marquette High School | 1:09.70 | Unverified: |
| 2 | TANZOLA, Audrey | SR | Marquette High School | 1:07.71 | Unverified: Rockwood Girls Quad |
| 3 | HALTER, FAITH | JR | Lafayette (Wildwood) High School | 1:06.70 | Verified |
| 4 | SCHNURBUSCH, Raegan | SO | Eureka High School | 1:03.50 | Unverified: |
| 5 | PAULS, Lauren | 9 | Lafayette (Wildwood) High School | 1:05.41 | Verified |
| 6 | POWELL, Emma | JR | Jackson High School | 1:07.51 | Verified |
| 7 | RYAN, Maegan | SR | Northwest (Cedar Hill) High Scho | 1:08.00 | Unverified: Kirkwood Early Bird |
| 8 | SIMON, Alise | SR | Francis Howell North High School | 1:10.02 | Unverified: |