| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | SOTO, Cristina | 9 | Lindbergh High School | NT | Unverified: |
| 3 | BUTLER, Ja'Naiya | 9 | Hazelwood Central High School | NT | Unverified: |
| 4 | JOHNSON, Adarah | 9 | Hazelwood Central High School | NT | Unverified: |
| 5 | KELLEY, Jeannette | 9 | Lindbergh High School | NT | Unverified: |
| 6 | RICHARDSON, Alana | SR | Riverview Gardens High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BLAND, McKenzie | 9 | Francis Howell North High School | NT | Unverified: |
| 2 | HARRIS, Kayla | 9 | Riverview Gardens High School | NT | Unverified: |
| 3 | PHILLIPS, Taylor | 9 | Parkway West High School | 1:27.00 | Unverified: |
| 4 | BRINER, AVA | SO | Jackson High School | 1:17.00 | Unverified: |
| 5 | ZIEGLER, Chloe | 9 | Northwest (Cedar Hill) High Scho | 1:17.00 | Unverified: Kirkwood Early Bird |
| 6 | CULBERT, Peyton | JR | Parkway West High School | 1:29.00 | Unverified: |
| 7 | MARSHALL, Makara | SO | McCluer High School | NT | Unverified: |
| 8 | DAVIS, Makaila | SO | Parkway North High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PILLAI, ARSHYA | 9 | Marquette High School | 1:14.50 | Unverified: |
| 2 | SMITH, Abriea | 9 | Parkway North High School | 1:11.53 | Verified |
| 3 | WALTERS, Olivia | SR | Marquette High School | 1:10.55 | Verified |
| 4 | MIMLITZ, Sophia | SR | Lafayette (Wildwood) High School | 1:08.40 | Verified |
| 5 | KILMER, KATE | SO | Lafayette (Wildwood) High School | 1:10.00 | Unverified: |
| 6 | CONLEY, Olivia | SO | Eureka High School | 1:11.50 | Unverified: |
| 7 | REED, Callista | JR | Eureka High School | 1:13.70 | Verified |
| 8 | WOOD, Jadyn | SR | Northwest (Cedar Hill) High Scho | 1:15.00 | Unverified: Kirkwood Early Bird |