| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Northwest (Cedar Hill) High Scho | A | Brandt | Gerber | Sullivan | Bounds | 48.72 | Verified |
| 4 | Riverview Gardens High School | A | Carlock | Crymes | Hodges | Howard | 46.76 | Verified |
| 5 | Notre Dame (Cape Girardeau) High | A | Adams | Eftink | Marshall | Stiegemeyer | 48.00 | Unverified: McCullough-Douglas Invitational |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jackson High School | A | Settle | Alley | Alspaugh | STRICKLAND | NT | Verified |
| 2 | Lafayette (Wildwood) High School | A | Marshall | LOCHMANN | Ems | Brodie | 45.58 | Unverified: |
| 3 | Marquette High School | A | BONNETT | ELLIS | CODY-JACKSON | Brown | 44.80 | Verified |
| 4 | Hazelwood Central High School | A | Banks | Martin | Williams | Harris | 43.53 | Verified |
| 5 | Parkway West High School | A | Harris | Bell | Campbell | Wayne | 44.00 | Unverified: |
| 6 | Francis Howell North High School | A | Kelly | McCrary | Stojanovic | Burris | 45.46 | Unverified: |
| 7 | Hazelwood East High School | A | Williams | Hill | Cain-Shockley | Sledge | 45.79 | Verified |
| 8 | McCluer High School | A | Reynolds | Bills | McCoy | Gillespie | 46.75 | Verified |