| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Northwest (Cedar Hill) High Scho | A | Grellner | Smith | Robinson | Herrington | NT | Verified |
| 4 | Francis Howell North High School | A | Hoskins | Dunseth | Hartley | Eiland | 47.46 | Verified |
| 5 | Jackson High School | A | HERRING | DRUM | COFFEY | CRUMP | 48.50 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Hazelwood East High School | A | Love | Johnson | Kirby | Alexander | 46.79 | Unverified: |
| 2 | McCluer High School | A | Jones | Johnson | Johnson | Guthrie | 46.75 | Verified |
| 3 | Kirkwood High School | A | Webb | Boswinkel | Smith | Marion | 46.50 | Unverified: |
| 4 | Hazelwood Central High School | A | Atkins | Thornton | Anthony | Harps | 45.34 | Unverified: |
| 5 | Lafayette (Wildwood) High School | A | Parks | KING | BERRA | STITH | 45.89 | Unverified: |
| 6 | Parkway North High School | A | Shaaban | Royal | Morgens-Clark | Smith | 46.62 | Unverified: |
| 7 | Riverview Gardens High School | A | Bibbs | Bowman | Rimmer | Jackson | NT | Verified |
| 8 | Marquette High School | A | JACKSON | NOLAN | JONES | TERRY | 46.80 | Unverified: Lindbergh Boys Flyer Track and F |