| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lindbergh High School | A | Hiers | Hertel | Thomas | Scott | NT | Unverified: |
| 4 | McCluer High School | A | Nashville | Hooks | Townsend | Pool | 1:02.57 | Verified |
| 5 | Oakville High School | A | Witte | Elza | Mueller | Beidle | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Notre Dame (Cape Girardeau) High | A | Huck | Tatum | Carr | Givens | 57.03 | Verified |
| 2 | Lafayette (Wildwood) High School | A | Halter | SADDLER | Shelton | White | 53.37 | Verified |
| 3 | Kirkwood High School | A | Burgett | Beaumont | Scully | Wilkes | 52.55 | Verified |
| 4 | Hazelwood Central High School | A | Mapp | Wilkins | Mckenzie | Brown | 50.72 | Unverified: |
| 5 | Eureka High School | A | DEAKIN | Schweizer | SEITZ | MITCHELL | 51.80 | Verified |
| 6 | Northwest (Cedar Hill) High Scho | A | Bertani | Cronin | Surratt | Brown | 53.00 | Unverified: Kirkwood Early Bird |
| 7 | Riverview Gardens High School | A | GLADDEN | Richardson | Smith | Smith | NT | Verified |
| 8 | Marquette High School | A | DAVIS | HOUCHIN | QIN | TROUPE | 57.93 | Verified |