| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Parkway North High School | A | McWilliams | Smith | Tillman | Hilton | NT | Unverified: |
| 4 | Northwest (Cedar Hill) High Scho | A | Haas | Bauwens | Amos | G'Sell | 59.90 | Unverified: Kirkwood Early Bird |
| 5 | McCluer High School | A | Nelson | Burr | Robinson | House | 1:02.57 | Verified |
| 6 | Lindbergh High School | A | Haviland | Gardner | Lord | Gonzalez | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Riverview Gardens High School | A | Tabron | harris | Long | Richardson | 58.00 | Unverified: |
| 2 | Lafayette (Wildwood) High School | A | WILDT | Vellines | Surber | Alvarez | 57.00 | Unverified: |
| 3 | Francis Howell North High School | A | Huff | Huff | Bland | StJohn | 55.65 | Verified |
| 4 | Eureka High School | A | Skaggs | KIEFER | BRICKMAN | DOSS | 54.00 | Unverified: |
| 5 | Hazelwood Central High School | A | Swanson | Witherspoon | Reece | Williams | 54.53 | Unverified: |
| 6 | Kirkwood High School | A | Sands | Wright | Cornelius | Perkins | 55.85 | Unverified: |
| 7 | Notre Dame (Cape Girardeau) High | A | Spooler | Page | Collins | Wagoner | 57.03 | Verified |
| 8 | Marquette High School | A | FLENOID | SONN | NEAL | BUNCH | 59.00 | Unverified: |