| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lindbergh High School | A | Hiers | Scott | Thomas | Kottmeier | NT | Unverified: |
| 4 | Oakville High School | A | Witte | JONAS | Mueller | Beidle | 2:50.00 | Unverified: |
| 5 | Parkway North High School | A | Easterwood | Nelson Grimes | Schrader | Davis | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marquette High School | A | Zweifel | Simmons | Mcgee | Arnold | 2:10.00 | Unverified: |
| 2 | Northwest (Cedar Hill) High Scho | A | Bertani | Shelledy | Surratt | Ryan | 2:05.00 | Unverified: |
| 3 | Jackson High School | A | Powell | Callis | Metzger | Metzger | 1:55.00 | Unverified: |
| 4 | Kirkwood High School | A | Beaumont | Scully | Dixon | Baker | 1:53.20 | Unverified: |
| 5 | Eureka High School | A | DEAKIN | SEITZ | Grobe | SCHNURBUSCH | 1:54.90 | Unverified: |
| 6 | Hazelwood Central High School | A | Union | Bufford | Jefferson | Wilkins | 2:00.40 | Unverified: |
| 7 | Lafayette (Wildwood) High School | A | SADDLER | White | MOULTON | Singh | 2:09.00 | Unverified: |
| 8 | Francis Howell North High School | A | Yauna Fullenwider | Simon | Chance | Jones | 2:10.06 | Unverified: |