| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lafayette (Wildwood) High School | A | Dantuono | Miriani | RINKENBERGER | Groenewald | 10:24.24 | Verified |
| 2 | Eureka High School | A | Holtman | Jones | HARTMANN | Christensen | 10:50.00 | Unverified: |
| 3 | Jackson High School | A | Golinski | GROSS | McBryde | Pope | 11:20.00 | Unverified: |
| 4 | Parkway North High School | A | Zheleva | Wong | Jones | Fish | 11:22.20 | Verified |
| 5 | Kirkwood High School | A | Clancy | Klein | Duggin | Canatsey | 12:01.00 | Unverified: |
| 6 | Hazelwood Central High School | A | Catching | Maxwell | Equere | Elam | 12:11.58 | Verified |
| 7 | Northwest (Cedar Hill) High Scho | A | Layton | Vandaveer | Kettmann | Brant | 12:16.00 | Unverified: Kirkwood Early Bird |
| 8 | Francis Howell North High School | A | Dustin | StJohn | Cash | Oser | 12:45.00 | Unverified: |
| 9 | Marquette High School | A | BOLT | Brandon | MARTIN | Wrisberg | 13:30.00 | Unverified: |
| 10 | Lindbergh High School | A | Kraft | Marsek | Hobson | Zoeller | NT | Unverified: |
| 11 | Parkway West High School | A | Etchason | Peters | Phung | Briner | NT | Unverified: |