| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fulton High School | A | Barnes | Monroe | Logan | Hutchins | 1:45.57 | Verified |
| 3 | Mexico High School | A | Shivers | DiBlasi | Ewing | Frazier | 1:34.93 | Verified |
| 4 | Hannibal High School | A | Humphrey | Robertson | Bergheger | Williams | 1:31.11 | Verified |
| 5 | Moberly High School | A | Borges | Winn | Rasico | Gelina | 1:34.63 | Verified |
| 6 | Kirksville High School | A | Farwell | Mendez | Novencido | Austic | 1:39.06 | Verified |
