| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Kirksville High School | A | Downen | Hicks | Starkey | Downen | 54.50 | Verified |
| 3 | Moberly High School | A | Fanning | Hannam | Messer | Ferguson | 53.38 | Verified |
| 4 | Hannibal High School | A | Williams | Dean | Stewart | LaJoy | 52.42 | Verified |
| 5 | Fulton High School | A | Kelly | Echelmeier | Begemann | Walton | 52.77 | Verified |
| 6 | Marshall High School | A | Neff | Thompson | Shepard | Lance | 53.56 | Verified |
