| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Moberly High School | A | Wilkey | Smothers | Rivera | Fenton | 11:11.66 | Verified |
| 2 | Hannibal High School | A | Griffen | Waelder | Bates | Bonvillian | 11:32.94 | Verified |
| 3 | Kirksville High School | A | McNeely | Sterup | Starkey | Melnick | 12:07.21 | Verified |
| 4 | Fulton High School | A | Schwinke | Connor | Boyd | Vanzetti | 12:22.33 | Verified |
