| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | GLADDEN, Mykale | JR | Riverview Gardens High School | NT | Unverified: |
| 4 | FRENCH, Mikel | JR | Ritenour High School | NT | Unverified: |
| 5 | MOORE, Latrisha | JR | McCluer North High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | PANUS, Paige | SR | Francis Howell High School | NT | Unverified: |
| 3 | WILLIAMS, Jordyn | SR | Ritenour High School | 59.54 | Verified |
| 4 | WILLIAMS, Justice | SR | McCluer North High School | 57.03 | Verified |
| 5 | EDWARDS, Kendall | SR | Hazelwood Central High School | 57.23 | Verified |
| 6 | PRICE, Kodie | SO | Hazelwood East High School | 1:04.00 | Unverified: |
| 7 | LUSTER, Samaya | SR | Carnahan High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIAMS, Kennedy | 9 | Hazelwood Central High School | 53.20 | Unverified: |
| 2 | RADFORD, Jada | JR | East St. Louis (Senior) HS | 52.20 | Unverified: |
| 3 | CADE, Kimmeran | SR | Metro High School | 51.90 | Verified |
| 5 | LEE, Sydney | SR | Hazelwood East High School | 49.12 | Unverified: |
| 6 | THOMAS, Jada | SO | East St. Louis (Senior) HS | 51.94 | Verified |
| 7 | SUTTON, Kelci | SR | Ritenour High School | 53.04 | Verified |
| 8 | REICHART, Brigid | SO | Francis Howell High School | 55.00 | Verified |
