| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | UNION, Journey | R.C Striders | NT | ||
| 4 | SIEMSEN, Zoe | Tallawah Track and Field | NT | ||
| 5 | MITCHELL, Jaela | K C Elite Competition | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | ELLISON, Caroline | Unattached | 28.30 | ||
| 3 | AUSTENE, Marissa | Blue Thunder Track Club | 27.40 | ||
| 4 | CRIS-KARGBO, Darlene | Capital Area Track 2 | 27.14 | ||
| 5 | MORRIS, Seleena | Capital Area Track 2 | 27.40 | ||
| 6 | TAYLOR, Mikyla | Kc Swag | 27.50 | ||
| 7 | WIEGAND, Avery | Unattached | 28.98 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REDMAN, Mirabella | Pony Express Athletic Performa | 27.00 | ||
| 2 | BOONE, Aseanti | Ultimate Speed Academy | 26.72 | ||
| 3 | DAVIS, Brooklyn | Palestine Prowlers Sports Asso | 26.14 | ||
| 4 | DIXON, Genesis | Missouri Elite Sports Club | 24.90 | ||
| 5 | RAGLIN, Renee | Blues Track Club of Greater St | 25.56 | ||
| 6 | MEDINA, Luciana | TRIBE | 26.50 | ||
| 7 | MAYO, Taylor | Springfield Track Club | 26.79 | ||
| 8 | COLE, Keiyah | Windsteppers | 27.12 |
