| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MORRIS, Seleena | Capital Area Track 2 | 2:27.00 | ||
| 2 | CASEY, Ansley | Blue Thunder Track Club | 2:29.00 | ||
| 3 | SINGER, Rachel | Tallawah Track and Field | 2:29.00 | ||
| 4 | BROWN, Makayla | Blues Track Club of Greater St | 2:39.06 | ||
| 5 | THOMPSON, Tessa | Excelerate Phenoms | 2:39.92 | ||
| 6 | KREEGER, Brooke | Unattached | 2:41.29 | ||
| 7 | WILKINS, Hadley | Tallawah Track and Field | NT |
