| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MUHWEZI, Nyeema | Team Wichita Elite | 2:19.00 | ||
| 2 | HURT, Lily | Pure Talent Elite Track and Fi | 2:26.00 | ||
| 3 | WILLIAMS, Lauryn | Excelerate Phenoms | 2:29.02 | ||
| 4 | UPTON, Jenna | St. Charles County Lightning | 2:30.00 | ||
| 5 | EATHERTON, Addison | Blue Thunder Track Club | 2:31.57 | ||
| 6 | JENKINS, Morgan | Capital Area Track 2 | 2:35.00 | ||
| 7 | COLEMAN, Lauryn | Excelerate Phenoms | 2:37.60 | ||
| 8 | NILLES, Alexis | Unattached | 2:41.00 | ||
| 9 | PICKETT, Emily | Blue Thunder Track Club | 2:50.00 | ||
| 10 | SPORNITZ, Abby | Olathe Elite TC | 3:21.09 | ||
| 11 | BORDE-KOUFIE, Danette | Capital Area Track 2 | NT | ||
| 12 | HILL, Alonna | Kc Swag | NT | ||
| 13 | WHITE, Damia | Herbert Hoover | NT |
