| Lane | Team | Squad |
|---|---|---|
| 1 | John Burroughs High School | A |
| 2 | Principia High School | A |
| 3 | Chaminade College Preparatory Sc | A |
| 4 | Hazelwood Central High School | A |
| 5 | Priory High School | A |
| 6 | MICDS | A |
| 7 | Whitfield High School | A |
| 8 | University City High School | A |
| 9 | Christian Brothers College High | A |
| 10 | Clayton High School | A |
| 11 | Lutheran North High School | A |
| 12 | Lutheran South High School | A |
| 13 | STEAM Academy at McCluer South-B | A |
| 14 | Affton High School | A |
| 15 | Gateway Science Charter | A |
| 16 | Lift for Life Academy Charter Hi | A |
