| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | KIPP St. Louis | B | NT | Verified | ||||
| 4 | Hancock High School | A | Britton | Stewart | Diggs | Martin | 1:50.65 | Verified |
| 5 | McCluer North High School | B | Coleman | Rimmer | Wilson | Davis | NT | Unverified: |
| 6 | Maplewood-Richmond Hts. High Sch | B | Leach | Brinkley | Gustin | Williams | 1:43.21 | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bayless High School | A | Miller | Saville | Chow | Patton | NT | Unverified: |
| 2 | Brentwood High School | A | Bradley | Chantharasy | Harris | Lane | NT | Unverified: |
| 3 | Metro High School | A | Fisher | Marley | Badillo | Dawson | 1:40.85 | Verified |
| 4 | KIPP St. Louis | A | Baker | Aziz | Grooms | Ray | 1:36.27 | Verified |
| 5 | Maplewood-Richmond Hts. High Sch | A | Hagens | Grawer | Posley | Shaw | 1:38.28 | Verified |
| 6 | Carnahan High School | A | Long | Lewis | Mull | Holmes | 1:43.64 | Verified |
| 7 | Orchard Farm High School | A | Lewis | Williams | Jewell | Pothoff | NT | Unverified: |
| 8 | McCluer North High School | A | Chandler | Gray | Hopgood | Kelley | NT | Unverified: |
