| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Maplewood-Richmond Hts. High Sch | A | Stewart | Robinson | Hurn | Kiernan | NT | Unverified: |
| 4 | Metro High School | A | Fuchs | Tate | Griffith | Anania | 1:59.71 | Verified |
| 5 | Orchard Farm High School | A | Ditmeyer | Shields | Jewell | Jones | NT | Unverified: |
| 6 | St. Charles West High School | A | Nichols | Boxdorfer | Zeier | Shivers | NT | Unverified: |
