| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | STECK, Anna | SR | Metro High School | 2:36.90 | Verified |
| 2 | LEFERS, Marissa | JR | Orchard Farm High School | 3:10.24 | Unverified: |
| 3 | PRITCHETT, Miranda | JR | Orchard Farm High School | 3:14.77 | Verified |
| 4 | TIBBS, Rachel | SO | Metro High School | 3:33.48 | Verified |
| 5 | MCCUNE, Betsy | SR | St. Charles West High School | NT | Unverified: |
| 6 | BRADFORD, Brianna | 9 | Bayless High School | NT | Unverified: |
| 7 | SENYARD, Sara | JR | St. Charles West High School | NT | Unverified: |
