| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JACKSON, Avery | SO | St. Charles West High School | NT | Unverified: |
| 2 | LEONARD, Abigail | SO | Metro High School | NT | Unverified: |
| 3 | BAER, Matilynn | JR | Orchard Farm High School | 1:33.83 | Verified |
| 4 | CHRISTIAN, Maeta | SR | Metro High School | 1:05.61 | Verified |
| 5 | WASHINGTON, Juanesha | SR | Carnahan High School | 1:15.59 | Verified |
| 6 | POWERS, Gabriel | JR | Brentwood High School | NT | Unverified: |
| 7 | RABB, Zeta | JR | Carnahan High School | NT | Unverified: |
| 8 | MOORE, Zena | 9 | Crossroads College Preparatory S | NT | Unverified: |
