| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Maplewood-Richmond Hts. High Sch | A | Robinson | Hurn | Brown | Harper-McCain | NT | Unverified: |
| 3 | Valley Park High School | A | Reeves | Miregwa | Lanasa | Reed | 1:02.87 | Verified |
| 4 | St. Charles West High School | A | Nichols | Myers | House | Myers | 53.10 | Unverified: |
| 5 | Metro High School | A | Fuchs | Tate | Jones | Griffith | 58.57 | Verified |
| 6 | Orchard Farm High School | A | Ditmeyer | Shields | Jewell | Jones | NT | Unverified: |
