| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Wellsville-Middletown High Schoo | A | Hollensteiner | Beach | Mayes | Seabaugh | 1:43.87 |
| 2 | Mark Twain High School | A | Lake | Miller | Lake | Jung | 1:40.85 |
| 3 | Louisiana High School | A | Greene | Luck | Washington | Chatman | 1:35.17 |
| 4 | Highland High School | A | Stegeman | Evans | Harmon | Stutsman | 1:35.30 |
| 5 | Missouri Military Academy | A | Ruckman | Anyaeto | Mutinyu | Schuh | 1:42.74 |
| 6 | Van-Far High School | A | Baugh | Woodson | Hartley | Walsh | 1:55.19 |
