| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Bowling Green High School | A | Henningfield | Summers | Mix | Wray | 4:04.00 |
| 2 | Missouri Military Academy | A | Canonico | Ruckman | Miles | Powell | DQ |
| 3 | Mark Twain High School | A | Northcutt | McLeod | Lake | Preston | 3:39.69 |
| 4 | Louisiana High School | A | Blackwell | Richards | Dzierwa | Harrison | 3:48.90 |
| 5 | Wellsville-Middletown High Schoo | A | Marshall | Mayes | Seabaugh | Beach | 4:01.47 |
| 6 | Highland High School | A | Geisendorfer | Harmon | May | Obrien | 4:20.02 |
