| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Clopton High School | A | Burt | Truetken | Martin | Cooke | 1:01.64 |
| 3 | Missouri Military Academy | A | Wang | Miles | Canonico | Ruckman | 52.22 |
| 4 | Van-Far High School | A | Baugh | Hartley | Woodson | Walsh | 54.91 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Mark Twain High School | A | Lake | Lake | Jung | Preston | DQ |
| 3 | Highland High School | A | Holder | Bringer | Goehl | Mallett | 44.94 |
| 4 | Louisiana High School | A | Greene | Richards | Luck | Chatman | 45.61 |
| 5 | Wellsville-Middletown High Schoo | A | Hollensteiner | Beach | Henderson | Slovensky | 51.29 |
