| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Clopton Middle School | A | Adam | Bankhead | Enloe | Caplin | 1:05.06 |
| 2 | North Callaway MS | A | Wilson | Slater | Schmidt | Horstman | 59.13 |
| 3 | Winfield MS | A | Holder | Liberson | Pund | VanCleve | 56.74 |
| 4 | Louisiana Middle School | A | Clark | Oliver | Rule | Griffith | 59.00 |
| 5 | Elsberry Middle School | A | Cecil | Glover | Risby | Sifuentes | 1:03.02 |
| 6 | Van-Far MS | A | Carmen | Brogden | Fraction | Keller | 1:07.69 |
