| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | La Plata High School | A | Mesecher | St. Clair | Wheeler | Jones | 1:43.58 | Verified |
| 4 | Northeast (Cairo) High School | A | Brockman | Hankins | Harman | Head | 1:38.84 | Verified |
| 5 | Kirksville High School | A | Mendez | Bedford | Novencido | Austic | 1:41.34 | Verified |
