| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | LUSHER, Florence | 9 | Schuyler County High School | NT | Unverified: |
| 4 | SHAHAN, Emily | SO | Schuyler County High School | NT | Unverified: |
| 5 | HALLEY, Anna | 9 | Novinger High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HARDEN, AVA | 9 | La Plata High School | 35.93 | Verified |
| 3 | TAYLOR, Morgan | SR | Northeast (Cairo) High School | 33.39 | Verified |
| 4 | ALMOND, Jordyn | SO | Novinger High School | 32.30 | Verified |
| 5 | MCNEELY, Ellen | SO | Kirksville High School | 32.88 | Verified |
| 6 | CLAY, Araya | 9 | Kirksville High School | 34.26 | Verified |
| 7 | RUMMERFIELD, Zailey | 9 | Kirksville High School | 35.48 | Verified |
| 8 | COY, Claire | SO | La Plata High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BURNS, Kennedy | SR | Schuyler County High School | 31.15 | Verified |
| 2 | BAILEY, Addison | 9 | Northeast (Cairo) High School | 30.49 | Verified |
| 3 | ELLISON, Caroline | JR | Northeast (Cairo) High School | 29.53 | Verified |
| 4 | LEWIS, Meranda | JR | Green City High School | 26.45 | Verified |
| 5 | DOWNEN, Phebe | JR | Kirksville High School | 29.40 | Verified |
| 6 | THOMAS, Maya | JR | Kirksville High School | 29.76 | Verified |
| 7 | STARKEY, Catherine | 9 | Kirksville High School | 30.97 | Verified |
| 8 | DOWNEN, Fanchine | SO | Kirksville High School | 31.74 | Verified |
