| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | CLAY, Araya | 9 | Kirksville High School | NT | Unverified: |
| 4 | LANHAM, Allie | SO | Schuyler County High School | NT | Unverified: |
| 5 | GOSSEEN, Kristen | 9 | Northeast (Cairo) High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TALLMAN, Kelsey | SO | Schuyler County High School | NT | Unverified: |
| 2 | HARDEN, AVA | 9 | La Plata High School | 1:16.96 | Verified |
| 3 | GORDON, Haylee | 9 | Schuyler County High School | 1:12.86 | Verified |
| 4 | LEE, Alexa | 9 | Green City High School | 1:11.82 | Verified |
| 5 | MCNEELY, Ellen | SO | Kirksville High School | 1:11.83 | Verified |
| 6 | LUSHER, Florence | 9 | Schuyler County High School | 1:13.53 | Verified |
| 7 | BORGMEYER, Alexa | SO | Brashear High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BAILEY, Jersey | SO | Northeast (Cairo) High School | 1:10.47 | Verified |
| 2 | CARVAJAL, Paige | SO | La Plata High School | 1:10.21 | Verified |
| 3 | HERBST, Jersey | SO | Kirksville High School | 1:06.84 | Verified |
| 4 | MORRIS, Jacie | JR | Schuyler County High School | 1:00.68 | Verified |
| 5 | HANNAM, Zoey | 9 | Moberly High School | 1:05.00 | Unverified: |
| 6 | SMITH, Carlee | SO | Kirksville High School | 1:08.40 | Unverified: Ken Asbury |
| 7 | ROBERTS, Hailey | 9 | Schuyler County High School | 1:11.56 | Verified |
