| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Schuyler County High School | A | Snider | Coombs | Cullers-Arnold | Humphrey | NT | Unverified: |
| 3 | La Plata High School | A | Mesecher | St. Clair | Green | Green | 48.98 | Verified |
| 4 | Putnam County High School | A | Lopez | Tomlin | Rouse | Perkins | 45.62 | Verified |
| 5 | Kirksville High School | A | Mendez | Austic | Lushiku | Omehele | 48.13 | Verified |
| 6 | Novinger High School | A | Farrell | Findling | Robinson | Kittle | NT | Unverified: |
