| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Schuyler County High School | A | Burns | Roberts | Tallman | Morris | NT | Unverified: |
| 3 | Brashear High School | A | White | Borgmeyer | Fisher | Borgmeyer | NT | Unverified: |
| 4 | Kirksville High School | A | Herbst | Mccarty | Smith | Lyons | 4:30.00 | Unverified: |
| 5 | Kirksville High School | B | Downen | Snyder | Adams | Clay | NT | Unverified: |
| 6 | Clark County High School | A | Ellison | Hess | WEAVER | Plenge | NT | Unverified: |
| 7 | Clark County High School | B | NT | Unverified: |
