| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | IRVIN, ISABELLA | 9 | Clark County High School | NT | Unverified: |
| 4 | LEE, Alexa | 9 | Green City High School | NT | Unverified: |
| 5 | CAMERON, ELEANOR | SR | Clark County High School | NT | Unverified: |
| 6 | ALMOND, Jordyn | SO | Novinger High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | RUMMERFIELD, Zailey | 9 | Kirksville High School | NT | Unverified: |
| 4 | FREDERICK, Kelsey | 9 | Novinger High School | 31.45 | Verified |
| 5 | ADAMS, Emma | 9 | Kirksville High School | NT | Unverified: |
| 6 | DOWNEN, Phebe | JR | Kirksville High School | NT | Unverified: |
| 7 | STARKEY, Catherine | 9 | Kirksville High School | NT | Unverified: |
| 8 | CLAY, Araya | 9 | Kirksville High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HICKS, Brooklyn | JR | Kirksville High School | 31.30 | Verified |
| 2 | PLENGE, Abby | JR | Clark County High School | 27.40 | Verified |
| 3 | SHINN, Whitney | JR | North Shelby High School | 26.89 | Verified |
| 4 | ELLISON, Alexis | SR | Clark County High School | 25.97 | Verified |
| 5 | LEWIS, Meranda | JR | Green City High School | 26.45 | Verified |
| 6 | MORRIS, Jacie | JR | Schuyler County High School | 26.94 | Verified |
| 7 | UHLMEYER, Addy | 9 | North Shelby High School | 30.50 | Unverified: |
| 8 | JONES, Emme | 9 | North Shelby High School | 31.44 | Unverified: Knox County JH Relays |
