| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JENNINGS, Patrick | SR | Kirksville High School | 2:17.90 | Verified |
| 2 | NICHOLS, Colin | SR | Clark County High School | 2:28.72 | Verified |
| 3 | MARLIN, Jack | SR | Kirksville High School | 2:34.84 | Verified |
| 4 | HATCHER, Kaden | SO | Green City High School | 2:41.82 | Verified |
| 5 | HIGGINS, John | 9 | Brashear High School | NT | Unverified: |
| 6 | HARRISON, Tristin | 9 | Green City High School | NT | Unverified: |
| 7 | NOE, Clancy | SO | Brashear High School | NT | Unverified: |
| 8 | KIMBRELL, Gabriel | 9 | Schuyler County High School | NT | Unverified: |
| 9 | FINDLING, Wes | JR | Novinger High School | NT | Unverified: |
| 10 | HOFFMEYER, Carter | 9 | Schuyler County High School | NT | Unverified: |
