| PL | Athlete | Yr. | Team | Time | HPL |
|---|---|---|---|---|---|
| 1 | SHINN, JONATHAN | SO | Clark County High School | 24.28 | 4 (1) |
| 2 | PEAVLER, Aaron | JR | Green City High School | 24.32 | 5 (1) |
| 3 | NOVENCIDO, Andrew | SO | Kirksville High School | 25.21 | 4 (2) |
| 4 | AUSTIC, Benjamin | SR | Kirksville High School | 25.46 | 4 (3) |
| 5 | MENDEZ, Cruz | 9 | Kirksville High School | 25.67 | 2 (1) |
| 6 | FREDRICKSON, Amos | SO | North Shelby High School | 25.73 | 5 (2) |
| 7 | FARWELL, Jackson | JR | Kirksville High School | 25.98 | 2 (2) |
| 8 | BEDFORD, Alepio | 9 | Kirksville High School | 26.14 | 2 (3) |
| 9 | KITTLE, Bryce | SO | Novinger High School | 26.29 | 5 (3) |
| 10 | SALAS, Xander | 9 | Green City High School | 26.33 | 1 (1) |
| 11 | AMERMAN, Samuel | SR | Kirksville High School | 26.45 | 2 (4) |
| 12 | DAY, Austin | 9 | Clark County High School | 26.56 | 1 (2) |
| 13 | JOHNSON, Kaden | SO | Green City High School | 26.61 | 4 (4) |
| 14 | OMEHELE, Ninove | JR | Kirksville High School | 26.62 | 3 (1) |
| 15 | DAVIS, Nolan | 9 | Kirksville High School | 26.84 | 2 (5) |
| 16 | MUKATU, Joel | SO | Kirksville High School | 26.90 | 1 (3) |
| 17 | SCHUETZ, Gavin | 9 | Clark County High School | 27.05 | 1 (4) |
| 18 | TIPTON, Tucker | 9 | Green City High School | 27.14 | 2 (6) |
| 19 | MENDEZ, Cristian | JR | Kirksville High School | 27.19 | 4 (5) |
| 20 | NOVENCIDO, Samuel | 9 | Kirksville High School | 27.25 | 4 (6) |
| 21 | ADAMS, Kash | 9 | Kirksville High School | 27.57 | 5 (4) |
| 22 | HINNERS, Maxwell | 9 | Schuyler County High School | 27.64 | 3 (2) |
| 23 | DAVIDSON, JOSHUA | SO | Clark County High School | 27.79 | 2 (7) |
| 24 | CULLERS-ARNOLD, Christopher | 9 | Schuyler County High School | 28.36 | 3 (3) |
| 25 | MCATEE, Koan | SO | Kirksville High School | 28.46 | 3 (4) |
| 26 | MANN, Matthew | SR | Clark County High School | 29.53 | 4 (7) |
| 27 | STERUP, Daniel | SO | Kirksville High School | 30.49 | 1 (5) |
| 28 | BURGESS, JERRET | 9 | Clark County High School | 30.59 | 3 (5) |
| 29 | SANDEFUR, Elliot | 9 | Kirksville High School | 31.07 | 3 (6) |
| 30 | CLARK, Sullivan | 9 | Kirksville High School | 31.39 | 3 (7) |
| 31 | WEERAWONG, AlongKorn | SO | Kirksville High School | 31.49 | 2 (8) |
| MUNSON, Jorden | 9 | Green City High School | NT | ||
| SIMMONS, Kellen | 9 | Kirksville High School | NT | ||
| SHAW, Anthony | JR | Brashear High School | NT | ||
| HAMNER, KADEN | SO | Clark County High School | NT | ||
| WHEELER, CALLAN | SO | Clark County High School | NT |
