| PL | Athlete | Yr. | Team | Time | HPL |
|---|---|---|---|---|---|
| 1 | WHEELER, CALLAN | SO | Clark County High School | 56.97 | 3 (1) |
| 2 | MENDEZ, Cruz | 9 | Kirksville High School | 57.72 | 3 (2) |
| 3 | JOHNSON, Kaden | SO | Green City High School | 1:00.80 | 3 (3) |
| 4 | SALAS, Xander | 9 | Green City High School | 1:00.99 | 2 (1) |
| 5 | MCATEE, Koan | SO | Kirksville High School | 1:02.17 | 3 (4) |
| 6 | FIELDS, Laydon | SR | Green City High School | 1:02.73 | 3 (5) |
| 7 | SIMMONS, Kellen | 9 | Kirksville High School | 1:03.64 | 2 (2) |
| 8 | HINNERS, Maxwell | 9 | Schuyler County High School | 1:03.73 | 2 (3) |
| 9 | NICHOLS, Colin | SR | Clark County High School | 1:03.93 | 1 (1) |
| 10 | SHAW, Anthony | JR | Brashear High School | 1:04.01 | 2 (4) |
| 11 | HATCHER, Kaden | SO | Green City High School | 1:05.47 | 3 (6) |
| 12 | COOMBS, Kaden | 9 | Schuyler County High School | 1:06.02 | 2 (5) |
| 13 | TIPTON, Tucker | 9 | Green City High School | 1:06.31 | 2 (6) |
| 14 | MANN, Matthew | SR | Clark County High School | 1:06.62 | 1 (2) |
| 15 | STERUP, Daniel | SO | Kirksville High School | 1:07.15 | 2 (7) |
| 16 | WEERAWONG, AlongKorn | SO | Kirksville High School | 1:09.89 | 1 (3) |
| 17 | BURGESS, JERRET | 9 | Clark County High School | 1:10.40 | 1 (4) |
| 18 | HIGGINS, John | 9 | Brashear High School | 1:11.30 | 2 (8) |
| SHINN, JONATHAN | SO | Clark County High School | NT | ||
| MUNSON, Jorden | 9 | Green City High School | NT |
