| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | THOMPSON, Emma | FR | Southern Indiana | 5:10.00 | |
| 2 | BROWNING, Morganne | SO | Arkansas Tech | 5:10.39 | Hendrix Invitational 2021 (3/14/2021) |
| 3 | SMITH, Madison | JR | Harding | 5:11.34 | NextEra Energy Harding Invitational (3/19/2021) |
| 4 | SYKORA, Mandy | UNA | UNAT-Arkansas Tech | 5:13.63 | 2021 Little Rock Invitational (4/10/2021) |
| 5 | MATHEWS, Kaitlyn | SR | Western Illinois | 5:14.05 | Summit League Outdoor Championships (5/13/2021) |
| 6 | GWALTNEY, Caroline | SO | SIU Edwardsville | 5:15.40 | |
| 7 | WALKER, Kaitlyn | SO | SIU Edwardsville | 6:01.24 | Billiken Invitational (3/26/2021) |
| 8 | CUDWORTH, Marcie | FR | Arkansas Tech | NT | |
| 10 | PROCOPIO, Faith | FR | Western Illinois | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TIREY, Kayla | JR | McKendree | 5:02.28 | McKendree Bearcat Classic (4/02/2021) |
| 2 | TREPTOW, Emily | FR | Memphis | 5:03.00 | |
| 3 | WOOD, Gabby | SR | SIU Edwardsville | 5:03.65 | OVC championship (5/13/2021) |
| 4 | HOLLIDAY, Victoria | FR | Tennessee-Martin | 5:05.00 | |
| 5 | MATUSIK, Sydney | SR | Southern Illinois | 5:05.00 | |
| 6 | MATHEWS, Elyse | SR | Western Illinois | 5:07.14 | Summit League Outdoor Championships (5/13/2021) |
| 7 | DOBBINS, Kaelyn | SO | Arkansas Tech | 5:09.01 | 2021 Great American Conference Track & Field Championships (4/15/2021) |
| 8 | BOST, Felicity | FR | Memphis | 5:10.00 | |
| 9 | NIESET, Brianna | FR | Southern Illinois | 5:10.00 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LEMKE, Emily | SR | SIU Edwardsville | 4:56.94 | Margaret Simmons Invite (3/25/2022) |
| 2 | KURFMAN, Lydia | FR | Memphis | 4:59.00 | |
| 3 | TEPE, Madelyn | SR | McKendree | 4:59.58 | McKendree Bearcat Classic (4/02/2021) |
| 4 | GILBERT, Erin | JR | Memphis | 4:59.83 | Crimson Tide Invitational (4/08/2021) |
| 5 | BROWN, Josie | JR | Southern Illinois | 5:00.00 | |
| 6 | KELLER, Maddie | JR | SE Missouri | 5:00.00 | |
| 7 | MULLER, Erika | FR | SE Missouri | 5:00.00 | |
| 8 | PUGH, Mackenzie | SO | SE Missouri | 5:00.00 | |
| 9 | MAYS, Ashlynn | SO | Arkansas Tech | 5:00.91 | 2021 Great American Conference Track & Field Championships (4/15/2021) |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WUESTEFELD, Paulina | SR | Western Illinois | 4:38.47 | Gibson Invitational sponsored by the Terre Haute CVB (4/16/2021) |
| 2 | EASTMAN, Hannah | SO | SE Missouri | 4:41.86 | |
| 3 | DEXHEIMER, Kassidy | SR | SIU Edwardsville | 4:41.88 | Sycamore Open (4/24/2021) |
| 4 | GRAY, Madalyn | FR | SE Missouri | 4:45.00 | |
| 5 | TREVENA, Madison | FR | Western Illinois | 4:45.00 | |
| 6 | LACHANCE, Blayne | SO | Tennessee-Martin | 4:53.94 | USD Early Bird (4/03/2021) |
| 7 | AMUNDSEN, Annie | FR | Tennessee-Martin | 4:55.00 | |
| 8 | BAILEY, Ansley | FR | Southern Illinois | 4:55.00 | |
| 9 | GOLDRING, Mackenzie | SR | Western Illinois | 4:56.30 | 2021 Kip Janvrin Open (4/23/2021) |
