| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Harding | D | NT | |||||
| 4 | SE Missouri | B | Perkins | Eftink | Sickles | Modrow | 3:30.00 | |
| 5 | SE Missouri | C | Bartlett | Sowerby | Lay | Little | 3:30.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | SE Missouri | D | Forrest | NT | ||||
| 2 | Western Illinois | B | Bokata | McKay | Lupo | Ortiz | 3:29.00 | If possibleC we would like the same section so we can see who the faster lead off split is. |
| 3 | Harding | C | Carnley | Ashmore | Brock | Laughary | 3:26.00 | |
| 4 | Chicago State | B | Spells | Smith | Jones | Kipchumba | NT | 2021 Joey Haines Invite (4/02/2021) |
| 5 | Tennessee-Martin | A | Askew | Martin | Cluver | Rone | 3:25.00 | |
| 6 | Tennessee-Martin | B | Cahill | Blyth | Davis | Glosser | 3:27.00 | |
| 7 | Southern Indiana | A | Pflanz | King | Garrett | McPheeters | 3:29.17 | GLVC Outdoor Championships (5/06/2021) |
| 8 | Tennessee-Martin | C | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Harding | B | Williams | Bush | Watson | Williams | 3:22.00 | |
| 2 | SE Missouri | A | Schwab | Garrett | Splitt | Crawford | 3:17.89 | |
| 3 | Southern Illinois | A | Sherrod | Hamilton | Rodgers | Williams | NT | Missouri Valley Championships (5/14/2021) |
| 4 | Harding | A | Gaye | Csokas | Kirby | Smith | 3:12.66 | 2022 94th Clyde Littlefield Texas Relays (3/23/2022) |
| 5 | Western Illinois | A | Brown | Lommel | Colley | Hofer | 3:12.86 | Summit League Outdoor Championships (5/13/2021) |
| 6 | Lewis | A | Wagner | Kocanda | DeBolt | Jones | 3:14.10 | St. Francis Fighting Chance Invite (5/14/2021) |
| 7 | McKendree | A | Bumpers | Walker | Jones | Kasinger | 3:19.40 | Dick Cochran Classic 2021 (4/23/2021) |
| 8 | Chicago State | A | Keith | Hubert | Bruce | Kiptanui | NT | 2021 Joey Haines Invite (4/02/2021) |
