| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Tennessee-Martin | C | Amundsen | Grandys | Nail | Roth | DNS | OVC Indoor Track & Field Championships (2/23/2022) |
| 4 | SE Missouri | C | Muller | Gordon | Eastman | Keller | 4:10.00 | |
| 5 | Tennessee-Martin | B | Holliday | Loyet | Lachance | Tankersley | 4:10.09 | OVC Indoor Track & Field Championships (2/23/2022) |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Western Illinois | B | Wuestefeld | Ford | Procopio | Trevena | 4:10.00 | |
| 2 | Murray State | C | Pauly | Fletcher | Vollet | Seeberger | 4:05.00 | |
| 3 | McKendree | A | Tepe | Searcy | Spates | Sarver | 4:03.80 | GLVC Outdoor Championships (5/06/2021) |
| 4 | Harding | B | Reid | Couey | Stramrood | Maples | NT | |
| 5 | Southern Indiana | A | Brown | Warren | Wittmer | Thompson | 4:03.49 | GLVC Outdoor Championships (5/06/2021) |
| 6 | Tennessee-Martin | A | Wyatt | Smith | Eimer | Chapman | 4:05.00 | |
| 7 | Harding | C | Hall | Hall | 4:06.00 | |||
| 8 | SE Missouri | B | Hardin | Smith | Boessen | Pugh | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Western Illinois | A | Harrison | Dure | Huston | Petrou | 4:01.41 | Alex Wilson Invitational (2/19/2022) |
| 2 | Lewis | A | Chalmers | Phillips | Mack | Winters | 3:55.00 | |
| 3 | Murray State | A | McSwain | Bangala | Evans | Hagans | 3:50.00 | |
| 4 | Southern Illinois | A | Pointer | Galmore | Garner | Custer | 3:42.04 | Missouri Valley Championships (5/14/2021) |
| 5 | Harding | A | Clerima | Szarzynska | Eakin | Shell | 3:48.86 | Pittsburg State University Tune-Up (4/24/2021) |
| 6 | SE Missouri | A | Fox | Morrill | Gray | Eakin | 3:53.27 | OVC Indoor Track & Field Championships (2/23/2022) |
| 7 | Murray State | B | Malone | James | Davis | Campbell | 3:57.00 | |
| 8 | Chicago State | A | Jepkoech | Forbes | Wilson | Wilson | NT | raw:4:01.161 2022 Western Athletic Conference Indoor T&F Championships (2/18/2022) |
