| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TOOLIE, Kai | SO | McKendree | NT | |
| 2 | BARTLETT, Xzavier | FR | SE Missouri | 1:02.98 | Rhodes College Track & Field Invitational (3/18/2022) |
| 3 | WILLIAMS, Jaylon | SR | Harding | 59.14 | Harding Invitational (3/18/2022) |
| 4 | FRY, Brady | JR | Tennessee-Martin | 58.15 | Margaret Simmons Invite (3/25/2022) |
| 5 | CARNLEY, Drake | FR | Harding | 58.59 | Harding Invitational (3/18/2022) |
| 6 | ROCKAITIS, Austin | FR | Lewis | 1:00.20 | Miner Invitational (3/26/2022) |
| 7 | WILLIAMS, Samuel | FR | Harding | 1:03.46 | Harding Invitational (3/18/2022) |
| 8 | BUMPERS, Johnathan | SR | McKendree | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BUSH, Savian | FR | Harding | 57.28 | 2022 OBU Invite (3/25/2022) |
| 2 | LAY, Dillon | SR | SE Missouri | 56.27 | OVC championship (5/13/2021) |
| 3 | WOOD, Brock | SO | SE Missouri | 53.22 | OVC championship (5/13/2021) |
| 4 | GAYE, Fallou | JR | Harding | 51.96 | Arkansas Twilight (5/07/2021) |
| 5 | CSOKAS, Nikolasz | SO | Harding | 52.29 | Arkansas Twilight (5/07/2021) |
| 6 | WAGNER, Connor | JR | Lewis | 54.19 | Benedictine University Invite (4/09/2021) |
| 7 | WALKER, Demetrius | SR | McKendree | 56.89 | Conference Carolinas Outdoor Track & Field Championships (4/23/2021) |
| 8 | MACK, Kevyere | FR | SIU Edwardsville | 57.99 | Margaret Simmons Invite (3/25/2022) |
