| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | BRUCE, Javon | Chicago State | NT | ||
| 4 | RONE, Chance | SR | Tennessee-Martin | 54.39 | 2022 Redhawks Invite (1/15/2022) |
| 5 | KIPTANUI, Brian Kiptanui | Chicago State | 54.75 | Aurora Grand Prix (1/29/2022) | |
| 6 | HUBERT, Lance | SO | Chicago State | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ALZOUBI, Sami | FR | Lewis | 53.21 | Miner Invitational (3/26/2022) |
| 2 | GRIFFIN, Henry | SO | Tennessee-Martin | 52.68 | Vanderbilt Black & Gold Invitational (4/16/2021) |
| 3 | GREAVES, Cole | Unattached | 51.80 | ||
| 4 | BARTLETT, Xzavier | FR | SE Missouri | 51.47 | OVC Indoor Track & Field Championships (2/23/2022) |
| 5 | SKINNER, Kenyon'TA | JR | Western Illinois | 51.50 | |
| 6 | MCKAY, Jayden | SO | Western Illinois | 52.58 | ESU Relays (3/24/2022) |
| 7 | CESALIEN, Olesson | SR | Chicago State | 52.95 | GVSU Snowdown Showdown (2/04/2022) |
| 8 | JONES, Joel | SO | Harding | 53.37 | Southern Arkansas University Dan Veach Invitational (3/26/2021) |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARTIN, Colby | SO | Tennessee-Martin | 50.71 | OVC championship (5/13/2021) |
| 2 | HAMILTON, Jeremiah | FR | Southern Illinois | 50.38 | 2022 MVC Indoor Track & Field Championship (2/27/2022) |
| 3 | RODGERS, Omar | SR | Southern Illinois | 49.78 | PNC Lenny Lyles Invitational (1/29/2022) |
| 4 | BRETZ, Brandon | JR | SIU Edwardsville | 47.83 | Gibson Invitational sponsored by the Terre Haute CVB (4/16/2021) |
| 5 | SCHWAB, Collin | SR | SE Missouri | 48.75 | OVC championship (5/13/2021) |
| 6 | JONES, Jacoby | JR | Lewis | 49.93 | GLVC Outdoor Championships (5/06/2021) |
| 7 | DEBOLT, Will | SO | Lewis | 50.65 | Miner Invitational (3/26/2022) |
| 8 | PERKINS, Austin | SR | SE Missouri | 51.23 |
