| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GANNINGER, Mikey | SO | Chaminade College Preparatory Sc | NT | |
| 2 | GARRETT, John | 9 | Vianney High School | 58.30 | Verified |
| 3 | STRONG, Antonio | 9 | St. Mary's (St. Louis) High Scho | 1:00.87 | Verified |
| 4 | REYNOLDS, Tyler | SO | Kirkwood High School | 56.26 | Verified |
| 5 | ROCHE, Andrew | SO | Vianney High School | 1:00.32 | Verified |
| 6 | HORTON, Tylon | 9 | St. Mary's (St. Louis) High Scho | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CRYMES, Chad | SR | Riverview Gardens High School | 54.67 | Verified |
| 2 | WEBB, Khalil | JR | Kirkwood High School | 54.00 | Unverified: |
| 3 | FIELDS, Derek | JR | Chaminade College Preparatory Sc | 55.20 | Verified |
| 4 | GRELLNER, Justin | SR | Northwest (Cedar Hill) High Scho | 53.29 | Verified |
| 5 | WASHINGTON, Devin | 9 | Northwest (Cedar Hill) High Scho | 54.10 | Unverified: |
| 6 | DAVIS, Jaylen | SR | Riverview Gardens High School | 55.15 | Unverified: |
