| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Vianney High School | A | 1:44.90 | Verified | ||||
| 3 | St. Louis University High School | A | 1:35.66 | Unverified: | ||||
| 4 | Northwest (Cedar Hill) High Scho | A | Brandt | Washington | Bounds | Herrington | 1:43.25 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Riverview Gardens High School | A | Carlock | Hodges | Howard | Jones | 1:35.14 | Verified |
| 3 | Kirkwood High School | A | Brown | Webb | Marion | Trawick | 1:30.80 | Verified |
| 4 | St. Mary's (St. Louis) High Scho | A | Strong | Green | Johnson III | Horton | 1:33.25 | Verified |
| 5 | Chaminade College Preparatory Sc | A | Cameron) Epps | Jenkins | Ward | Griffin | 1:35.40 | Unverified: Chaminade Tri |
